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Image Search Results
Journal: Cancer research
Article Title: Quantitative fluorescent microscopy measures vascular pore size in primary and metastatic brain tumors
doi: 10.1158/0008-5472.CAN-16-1711
Figure Lengend Snippet: Representative images from the simultaneous injection of 14C-AIB with TX Red 625Da are shown. A is the eGFP RG-2 tumor and B is the terminal accumulation of TX Red 625Da. The accumulation of dye is then converted to an intensity image where red is the highest areas of dye accumulation and blue is the lowest (C). This data is then directly compared to the 14C-AIB autoradiogram (D) which shows similar degrees of accumulation of tracer. This experiment provided concurrent QAR and fluorescent data which allows a direct comparison of permeability variances. Multiple time point graphical analysis of individual experiments to calculate the transfer constant Kin, in normal brain (open circles) and tumor (filled circles) for TX Red 625Da (red dots) and 14C-AIB (blue dots) are shown in E and F respectively. The slope of the regressed line is equivalent to Kin and the ordinate intercept is equivalent to the vascular volume of the tracer; Vi. A linear regressed line was the best fit for the data (goodness of fit; quantification of sum of squares). Scale bar represents 1mm. Blood to brain transfer coefficients for AIB, TX Red 625Da, TX Red 3kDa, and TX Red 70kDa in brain, and the tumor are shown in Fig 2G. The Kin values were obtained from individual multiple time point graphical analysis plots in each respective regions of interest. A ** and *** indicates a p value of <0.01 and <0.001 respectively. Data are mean ± SD; n=3-6 for all data points.
Article Snippet: Briefly male Ficher-344 rats (
Techniques: Injection, Permeability